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spsK [2019-06-27 16:31:20]
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spsK [2019-06-27 16:31:20]

dTDP-4-dehydrorhamnose reductase, spore coat polysaccharide synthesis
locus
BSU37820
pI
6.08
mw
31.43 kDa
protein length
283 aa Sequence Blast
gene length
852 bp Sequence Blast
function
rhamnose biosynthesis, spore coat polysaccharide synthesis
product
dTDP-4-dehydrorhamnose reductase
essential
no
ec
1.1.1.133
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.7|Biosynthesis of rhamnose (for the exosporium)]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    3,883,427 3,884,278

    The protein

    Protein family

  • dTDP-4-dehydrorhamnose reductase family (according to Swiss-Prot)

    • Structure

  • [PDB|3SC6] (dTDP-4-dehydrorhamnose reductase (''rfbD'') from ''Bacillus anthracis'' str. Ames in complex with NADP; 42% identity, 72% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • BKE37820 ([gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACCTTTGTCAATCAGATCA, downstream forward: _UP4_TATTTGCAGGAAAGGAGCAG
  • BKK37820 ([gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACCTTTGTCAATCAGATCA, downstream forward: _UP4_TATTTGCAGGAAAGGAGCAG

    • References

  • 9353933,15383836,22960854,25239894,26577401